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MEDICAL STUDIES CANCER AND DIABETES-CLICK HERE TO VIEW THE IONIZER UNIT

MEDICAL STUDIES CANCER AND DIABETES
REDUCED WATER FOR PREVENTION OF DISEASE
DR. SANETAKA SHIRAHATA

Note: cancer suppression and insulin-like activity of "reduced" (ionized) water see below

Publication Biochemical and biophysical research communications
Volume 234, Issue 1, Pages 269-74
Date May 8, 1997
Journal Abbr Biochem Biophys Res Commun
ISSN 0006291
Repository NCBI PubMed
Extra PMID: 9169001
Date Added Monday, May 12, 2008 4:11:04 PM
Modified Monday, May 12, 2008 4:11:04 PM
Tags:
Ascorbic Acid, Catalase, Chemiluminescent Measurements, Copper, DNA Damage, Electrochemistry, Electrolysis, Electrophoresis, Agar Gel, Free Radical Scavengers, Free Radicals, Hydrogen, Hydrogen-Ion Concentration, Nitrogen, Oxidation-Reduction, Oxygen, Reactive Oxygen Species, Water, Xanthine Oxidase.
Notes:
Reduced Water For Prevention of Disease
Dr.Sanetaka Shirahata
Graduate school of Genetic Resources Technology, Kyushu University,
6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.

It has long been established that Reactive Oxygen Species (ROS) cause many types of damage to biomolecules and cellular structures, that, in turn result in the development of a variety of pathologic states such as diabetes, cancer and aging. Reduced water is defined as anti-oxidative water produced by reduction of water. Electrolyzed reduced water (ERW) has been demonstrated to be hydrogen-rich water and can scavenge ROS in vitro (Shirahata et al., 1997). The reduction of proton in water to active hydrogen (atomic hydrogen, hydrogen radical) that can scavenge ROS is very easily caused by a weak current, compared to oxidation of hydroxyl ion to oxygen molecule. Activation of water by magnetic field, collision, minerals etc. will also produce reduced water containing active hydrogen and/or hydrogen molecule. Several natural waters such as Hita Tenryosui water drawn from deep underground in Hita city in Japan, Nordenau water in Germany and Tlacote water in Mexico are known to alleviate various diseases. We have developed a sensitive method by which we can detect active hydrogen existing in reduced water, and have demonstrated that not only ERW but also natural reduced waters described above contain active hydrogen and scavenge ROS in cultured cells.

Cancer cells are generally exposed to high oxidative stress. Reduced water cause impaired tumor phenotypes of human cancer cells, such as reduced growth rate, morphological changes, reduced colony formation ability in soft agar, passage number-dependent telomere shortening, reduced binding abilities of telomere binding proteins and suppressed metastasis. Reduced water suppressed the growth of cancer cells transplanted into mice, demonstrating their anti-cancer effects in vivo.

(Diabetes)
ROS is known to cause reduction of glucose uptake by inhibiting the insulin-signaling pathway in cultured cells. Reduced water scavenged intracellular ROS and stimulated glucose uptake in the presence or absence of insulin in both rat L6 skeletal muscle cells and mouse 3T3/L1 adipocytes. This insulin-like activity of reduced water was inhibited by wortmannin that is specific inhibitor of PI-3 kinase, a key molecule in insulin signaling pathways. Reduced water protected insulin-responsive cells from sugar toxicity and improved the damaged sugar tolerance of type 2 diabetes model mice, suggesting that reduced water may improve insulin-independent diabetes mellitus.

Reduced water will be applicable to not only medicine but also food industries, agriculture, and manufacturing industries.

Shirahata, S. et al.: Electrolyzed reduced water scavenges active oxygen species and protects DNA from oxidative damage. Biochem. Biophys. Res. Commun., 234, 269174, 1997
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